Ethanolic root and shoot extracts of S. nigrum plants were used for assaying free amino acids (FAAs) levels using a Waters Acquity UPLC-tqd system (Milford, MA, United States) equipped with a BEH amide 2.1 × 50 column according to (Sinha et al., 2013 (link)) with the minor modification previously described by (AbdElgawad et al., 2015 (link)).
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