Profiling Adipocyte-Derived Extracellular Vesicles

Adipocyte-derived EVs were isolated using the commercially available EoxQuick Precipitation Solution (System Biosciences, Mountain View, CA) from the serum of an all-female subset, chosen to be phenotypically representative of the larger cohort, as previously described [15 (link)]. Total RNA was extracted from adipocyte-derived EVs using the commercially available SeraMir Exosome RNA Amplification Kit (System Biosciences, Mountain View, CA) according to manufacturer instructions. RNA was labeled with Affymetrix^® FlashTag™ Biotin HSR RNA Labeling Kit (Affymetrix, Santa Clara, CA) according to standard procedures. Labeled RNA was hybridized to Affymetrix GeneChip microRNA 4.0 arrays and run using a Fluidics Station 450 Protocol (FS450_002) (Affymetrix, Santa Clara, CA). MicroRNAs and ProbeIDs used for statistical analysis are provided in Additional file 2: Table S1 (Accession #: GSE125494).

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Barberio M.D., Kasselman L.J., Playford M.P., Epstein S.B., Renna H.A., Goldberg M., DeLeon J., Voloshyna I., Barlev A., Salama M., Ferrante S.C., Nadler E.P., Mehta N., Reiss A.B, & Freishtat R.J. (2019). Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs. Journal of Translational Medicine, 17, 232.

Publication 2019

SeraMir Exosome RNA Amplification Kit FlashTag™ Biotin HSR RNA Labeling Kit GeneChip microRNA 4.0 arrays

Adipocyte Biotin Exosome Female Genechip Micrornas Serum

Corresponding Organization :

Other organizations : Children's National, Winthrop-University Hospital, National Heart Lung and Blood Institute

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

MicroRNA expression in adipocyte-derived extracellular vesicles (EVs)

control variables

All-female subset chosen to be phenotypically representative of the larger cohort

controls

Positive control: None mentioned
Negative control: None mentioned

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