Effects of Arsenic on Adipose-Derived Stem Cells

The human prostate cancer cell line PC3 was obtained from American Type Culture Collection and maintained in 10% fetal bovine serum (FBS, Fisher) in Roswell Park Memorial Institute medium 1640 (RPMI, Corning). ASCs from two male Caucasian donors, generously provided by J. Gimble (Tulane University), were collected as previously described (Zolochevska et al. 2014 (link)) and cultured on fibronectin-coated plasticware in modified media conditions that have been described previously (Ruiz et al. 2010 (link)). ASCs from Donor 1 were isolated from midsection liposuction, and Donor 2 ASCs were isolated from liposuction samples from breast tissue. Briefly, “ASC media” was prepared to a final concentration of 60% Dulbecco’s Modified Eagle Medium (DMEM, Corning), 40% MCDB-201 medium (Sigma), 5% FBS, 1× insulin–transferrin–selenium (Corning), 1 nM dexamethasone (Acros Organics), 10 ng/mL epidermal growth factor (eBioscience), 0.1 μM ascorbic acid (Acros Organics), and 1× antibiotic–antimycotic (Gibco). For iAs exposure, culture medium with 0, 1, 10, or 75 ppb iAs (sodium arsenite, RICCA) was used. All ASC experiments used cells with a passage number < 10.