Fractionation of Urine DNA

Freshly collected urine was immediately mixed with 0.5 mol/L EDTA, pH 8.0, to a final concentration of 10 mmol/L EDTA and stored at − 20 °C. Total urine DNA was isolated by adding an equal volume of 6 mol/L guanidine thiocyanate (Sigma, St. Louis, MO) to thawed urine as described previously [17 (link)]. The fractionated high molecular weight (HMW) urine DNA (> 1 kb) and low molecular weight (LMW) urine DNA (≤1 kb) was obtained from total urine DNA using carboxylated magnetic beads (Agentcourt Bioscience Corporation, Beverly, MA), as previously described [22 (link)].

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Jain S., Su Y.H., Su Y.P., McCloud S., Xue R., Lee T.J., Lin S.C., Lin S.Y., Song W., Steffen J.D, & Hu C.T. (2018). Characterization of the hepatitis B virus DNA detected in urine of chronic hepatitis B patients. BMC Gastroenterology, 18, 40.

Publication 2018

guanidine thiocyanate

Edta Guanidine thiocyanate Urine

Corresponding Organization :

Other organizations : JBS Science (United States), Baruch S. Blumberg Institute, Yuncheng University, Tzu Chi University, Buddhist Tzu Chi General Hospital, Doylestown Hospital, Screen

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Variable analysis

independent variables

Mixing freshly collected urine with 0.5 mol/L EDTA, pH 8.0, to a final concentration of 10 mmol/L EDTA

dependent variables

Total urine DNA
Fractionated high molecular weight (HMW) urine DNA (> 1 kb)
Fractionated low molecular weight (LMW) urine DNA (≤1 kb)

control variables

Storage of the urine-EDTA mixture at − 20 °C

controls

No positive or negative controls were explicitly mentioned in the provided input.

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