Drosophila Genetic Manipulation Protocols

All Drosophila stocks and crosses were fed with malt and semolina-based food with 12 h light and dark daily rhythms at 25°C with 60% humidity, unless mentioned otherwise. Drosophila strains used in this study were UAS-da^RNAi GD51297 and UAS-da^RNAi KK105258 from the Vienna Drosophila Resource Center, CantonS (a gift from Dr Bertram Gerber, Leibniz Institute for Neurobiology, Magdeburg, Germany), da^G32-Gal4 (a gift from Riitta Lindström, University of Helsinki, Helsinki, Finland), UAS-TCF4B (Tamberg et al., 2015 (link)), 201Y-Gal4 [Bloomington Drosophila Stock Center (BDSC, 4440)], 30Y-Gal4 (BDSC, 30818) (Yao Yang et al., 1995 (link)) and OK107-Gal4 (BDSC, 854) were gifts from Mark Fortini, Thomas Jefferson University, Philadelphia, PA, USA. R12B08-Gal4 (BDSC, 48489) (Pfeiffer et al., 2008 (link), flweb.janelia.org/cgi-bin/view_flew_imagery.cgi?line=R12B08), elav-Gal4 (BDSC, 8760) (Luo et al., 1994 (link)), UAS-Dcr2;Pin¹/CyO (BDSC, 24644) (Dietzl et al., 2007 (link)), UAS-nls-GFP (BDSC, 4776), UAS-da^G (BDSC, 37291), UAS-mCD8-GFP; Pin¹/CyO (BDSC, 5136) and da mutant line da¹⁰ (BDSC, 5531) (Caudy et al., 1988 (link)) were obtained from the BDSC. The following transgenic lines were generated in this study: 3xFLAG-da^2M4 and sfGFP-da^4M1.

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Tamberg L., Jaago M., Säälik K., Sirp A., Tuvikene J., Shubina A., Kiir C.S., Nurm K., Sepp M., Timmusk T, & Palgi M. (2020). Daughterless, the Drosophila orthologue of TCF4, is required for associative learning and maintenance of the synaptic proteome. Disease Models & Mechanisms, 13(7), dmm042747.

Publication 2020

201Y-Gal4 30Y-Gal4

Da10 Dcr2 Drosophila Flew Food Gifts Humidity Imagery Light Pin1 Strains Transgenic

Corresponding Organization :

Other organizations : Tallinn University of Technology, Protobios (Estonia)

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Variable analysis

independent variables

UAS-da^RNAi^ GD51297
UAS-da^RNAi^ KK105258
UAS-TCF4B
3xFLAG-da^2M4^
SfGFP-da^4M1^

dependent variables

Not explicitly mentioned

control variables

CantonS
Da^G32^-Gal4
201Y-Gal4
30Y-Gal4
OK107-Gal4
R12B08-Gal4
Elav-Gal4
UAS-Dcr2;Pin^1^/CyO
UAS-nls-GFP
UAS-da^G^
UAS-mCD8-GFP; Pin^1^/CyO
Da mutant line da^10^

controls

CantonS (a gift from Dr Bertram Gerber, Leibniz Institute for Neurobiology, Magdeburg, Germany)
Da^G32^-Gal4 (a gift from Riitta Lindström, University of Helsinki, Helsinki, Finland)
UAS-TCF4B (Tamberg et al., 2015 (link))
201Y-Gal4 [Bloomington Drosophila Stock Center (BDSC, 4440)]
30Y-Gal4 (BDSC, 30818) (Yao Yang et al., 1995 (link))
OK107-Gal4 (BDSC, 854) (gifts from Mark Fortini, Thomas Jefferson University, Philadelphia, PA, USA)
R12B08-Gal4 (BDSC, 48489) (Pfeiffer et al., 2008 (link), flweb.janelia.org/cgi-bin/view_flew_imagery.cgi?line=R12B08)
Elav-Gal4 (BDSC, 8760) (Luo et al., 1994 (link))
UAS-Dcr2;Pin^1^/CyO (BDSC, 24644) (Dietzl et al., 2007 (link))
UAS-nls-GFP (BDSC, 4776)
UAS-da^G^ (BDSC, 37291)
UAS-mCD8-GFP; Pin^1^/CyO (BDSC, 5136)
Da mutant line da^10^ (BDSC, 5531) (Caudy et al., 1988 (link))

Annotations

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