HEPES, ethylenediaminetetraacetate (EDTA), bovine serum albumin (BSA), penicillin, streptomycin, dihydrorhodamine 123 (DHR), phenylmethylsulfonyl fluoride (PMSF), benzamidine, leupeptin, and soybean trypsin inhibitor (SBTI), sodium orthovanadate (Na3VO4), apocynin (4-acetovanillone), 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenol tetrazolium bromide (MTT), pyrrolidine dithiocarbamate (PDTC), diphenyleneiodonium (DPI), TRITC-labelled phalloidin, cycloheximide (CHX), ribonuclease A (RNase A), propidium iodide (PI), diethylenetriamine pentaacetic acid (DTPA), RPMI-1640 medium, superoxide dismutase conjugated to polyethylene glycol (PEG-SOD), catalase conjugated to PEG (PEG-CAT) and sulforhodamine B were from Sigma-Aldrich (St. Louis, MO). Triton X-100, Percoll, PVDF membranes, Rainbow™ were from GE Healthcare (San Francisco, CA). Fetal bovine serum (FBS) was purchased from Cultilab (Campinas, SP, Brazil). Dulbecco's Modified Eagle Medium (DMEM), Dihydroethidium (DHE), anti- p-FAK397 and Lipofectamine 2000 were obtained from Invitrogen (Carlsbad, CA). DAF-FMDA, CM-H2DCFDA, HPF and JC-1 were obtained from Molecular Probes (Carlsbad, CA). siRNA oligomers for NOX4 (5′-CCTCAGCATCTGTTCTTAACCTCAA-3′) and its scrambled sequence (Scramble) were obtained using BLOCKiTTM RNAi Designer (Invitrogen). Antibody anti-caspase-3 was obtained from Cell Signaling. All other antibodies and protein A/G agarose were from Santa Cruz Biotechnology (Santa Cruz, CA). Streptavidin-conjugated FITC and Streptavidin-conjugated horseradish peroxidase were from Caltag Laboratories. ECL system (SuperSignal West Pico chemiluminescent substrate kit) was from Pierce Biotechnology (Rockford, IL, USA). High capacity cDNA reverse transcripition kit RNeasy, RNeasy Mini kit were from Qiagen, RQ1 RNase-Free DNase, the set of dN TP and RNasin RNase inhibitor were purchased from Promega (Madison, WI).
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