Botryococcus braunii samples were isolated
from the fresh waters of Tenggarong, Kutai Kartanegara Regency, East
Kalimantan, Indonesia. The green microalgae were then cultivated at
the Laboratory of Animal Physiology, Developmental and Molecular Animals,
Faculty of Mathematics and Natural Sciences, Mulawarman University,
Indonesia, using the method described in our previous paper.39 (link) After harvesting, the algal paste was centrifuged
for 30 s at a speed of 5000 rpm. Following that, the algal samples
were homogenized for 30 s at a speed of 300 rpm and dried for 24 h
in a freeze-dryer. The freeze-dried samples were kept at 20 °C.
The water content, ash content, protein content, total chlorophyll,
and fatty acid composition of the freeze-dried samples were analyzed
by the methods described in our previous study.39 (link) The physical and chemical properties of Botryococcus
braunii are presented inTable 1 .
from the fresh waters of Tenggarong, Kutai Kartanegara Regency, East
Kalimantan, Indonesia. The green microalgae were then cultivated at
the Laboratory of Animal Physiology, Developmental and Molecular Animals,
Faculty of Mathematics and Natural Sciences, Mulawarman University,
Indonesia, using the method described in our previous paper.39 (link) After harvesting, the algal paste was centrifuged
for 30 s at a speed of 5000 rpm. Following that, the algal samples
were homogenized for 30 s at a speed of 300 rpm and dried for 24 h
in a freeze-dryer. The freeze-dried samples were kept at 20 °C.
The water content, ash content, protein content, total chlorophyll,
and fatty acid composition of the freeze-dried samples were analyzed
by the methods described in our previous study.39 (link) The physical and chemical properties of Botryococcus
braunii are presented in
