Genetic Mouse Models for Disease Research

C57BL/6 wild-type, Saa^−/−, Lrp1^fl/fl, and Lrp1^ΔCd11c mice were bred and maintained in the barrier facility at the University of Texas Southwestern Medical Center. Saa^−/− mice were generated using CRISPR/Cas9 genome editing on a C57BL/6J background (15 (link)). Lrp1^fl/fl mice (46 (link)) on a 129S7/SvEvBrd genetic background were used to generate a CD11c⁺ cell-specific Lrp1 knockout (Lrp1^ΔCd11c) mouse by crossing with a mouse expressing Cre recombinase under the control of the CD11c promoter (Jackson Laboratories; B6.Cg-Tg (Itgax-cre)1-1Reiz/J). Mice 8–12 weeks of age were used for all experiments and co-housed littermates were used as controls. Experiments were performed using protocols approved by the Institutional Animal Care and Use Committees of the UT Southwestern Medical Center.