Western Blot Antibody Reagents and Protocols

Anti-FLAG mouse monoclonal antibody was purchased from Stratagene (Cat # 200472). Anti-HA mouse monoclonal antibody was purchased from Thermo Fisher Scientific (Cat #26183). Anti-GAPDH, HRP-conjugated antibody was purchased from Santa Cruz Biotechnologies (Cat #sc-365062 HRP). Goat anti-mouse and anti-rabbit HRP conjugated secondary antibodies were purchased from Azure Biosystems (Cat #AC2115) or Cell Signaling Technologies (Cat #7074S), respectively. Antibodies raised against VACV proteins A17 and A10 were gifts from Dr. Bernard Moss [65 (link)–67 (link)]. Antibodies raised against VACV protein E3 were a gift from Dr. Yan Xiang [68 (link)]. Cycloheximide(Cat#01810) was purchased from Sigma. Phenol:Chloroform:Isoamyl Alcohol (Cat#15593031) was purchased from Invitrogen. qPCR mix (Cat#QP005) was purchased from GeneCopoeia. SuperScript III Reverse Transcriptase (Cat#18-080-044), Trizol reagent (Cat#15-596-018), RNase inhibitor (Cat#AM2696), DTT (Cat#BP172) and D-Sucrose (Cat#BP220-212) were purchased from Fisher Scientific.

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Cantu F., Cao S., Hernandez C., Dhungel P., Spradlin J, & Yang Z. (2020). Poxvirus-encoded decapping enzymes promote selective translation of viral mRNAs. PLoS Pathogens, 16(10), e1008926.

Publication 2020

Anti-HA mouse monoclonal antibody Cycloheximide Phenol:Chloroform:Isoamyl Alcohol qPCR mix SuperScript III Reverse Transcriptase Trizol reagent RNase inhibitor DTT D-Sucrose

Anti antibody Antibodies Antibody Azure Chloroform Cycloheximide E3 protein Gapdh Gifts Goat Isoamyl alcohol Moss Mouse Phenol Proteins Rabbit Reverse transcriptase Rnase Sucrose Trizol

Corresponding Organization : Kansas State University

Top 5 similar protocols

Variable analysis

independent variables

Anti-FLAG mouse monoclonal antibody
Anti-HA mouse monoclonal antibody
Anti-GAPDH, HRP-conjugated antibody
Goat anti-mouse and anti-rabbit HRP conjugated secondary antibodies
Antibodies raised against VACV proteins A17, A10, and E3
Cycloheximide

dependent variables

Not explicitly mentioned

control variables

Phenol:Chloroform:Isoamyl Alcohol
QPCR mix
SuperScript III Reverse Transcriptase
Trizol reagent
RNase inhibitor
D-Sucrose

controls

Antibodies raised against VACV proteins A17, A10, and E3
Not explicitly mentioned

Annotations

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