Quantifying Cellular Antioxidant and Lipid Peroxidation

GSH is an important antioxidant, and the depletion of GSH can trigger ferroptosis [39 (link)]. Malondialdehyde (MDA) is an end-product of lipid peroxides, which is used to assess ferroptosis [40 (link)]. The cellular or tissue GSH, MDA content, tissue iron levels and serum iron levels were detected using the reduced glutathione (GSH) assay kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), the malondialdehyde (MDA) detection kit (S0131, Beyotime Biotechnology, Shanghai, China), the tissue iron assay kit (A039-2-1) and the serum iron assay kit (A039-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s instructions. The concentrations of GSH and MDA were determined by measuring the absorbance at 405 nm and 530 nm. The values for the levels of tissue and serum iron were examined at a wavelength of 520 nm.