At the end of experiment, for identification of MCs, lung tissue sections (7 μm) were stained with toluidine blue, as described previously [63 (link)]. Every section was observed at a magnification of 100×, using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) associated with Leica LAS X Navigator software (Leica Microsystems SpA, Milan, Italy).
Free full text: Click here