Wheat cDNA Probes Mapped by FISH

We selected 45 cDNA probes previously mapped by single-gene FISH to bread wheat chromosomes by Danilova et al. (2014 (link)). The cDNA clones were developed by the National BioResource Project-Wheat, Japan. One sequence; the Acc2 cDNA pooled probe included in this study was developed from wheat RNA by Danilova et al. (2012 (link)). All cDNA clones were kindly supplied by the Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University. cDNA sequences were amplified using PCR with T3/T7 primers and LongAmp DNA Polymerase (New England Biolabs, Massachusetts, USA) following the manufacturer’s recommendations. The PCR products were purified with the Invitrogen PCR purification kit (Life Technologies, Carlsbad, USA) according to the manufacturer’s instructions.