Colorimetric Determination of Anthocyanins

The determination of the total anthocyanins was carried out by a colorimetric method [24 (link),25 (link)]. Spectrophotometric analyses were performed under the following conditions: to 5 mL of liquor were added 40 mL of an EtOH/HCl mixture previously prepared by mixing 79.3 mL of anhydrous ethyl alcohol with 20.3 mL of HCl (37%). The absorbance was measured at 535 nm by using the UV-160A Shimadzu instrument. The calibration curve was obtained by measuring the absorbance of standard solutions of pure cyanidin-3 glucoside. The anthocyanin concentrations were calculated using the extinction coefficient 1018.3. Measurements were made in triplicate. Standard deviations (SD) were within ±2%.

Free full text: Click here

Cassano A., Conidi C, & Ruby-Figueroa R. (2014). Recovery of Flavonoids from Orange Press Liquor by an Integrated Membrane Process. Membranes, 4(3), 509-524.

Publication 2014

UV-160A

Anhydrous ethyl alcohol Anthocyanin Colorimetric Cyanidin 3 glucoside Etoh Extinction Liquor Spectrophotometric

Corresponding Organization :

Other organizations : Institute on Membrane Technology, University of Calabria

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total anthocyanins

control variables

Volume of liquor (5 mL)
Composition of EtOH/HCl mixture (79.3 mL anhydrous ethyl alcohol, 20.3 mL HCl (37%))
Wavelength for absorbance measurement (535 nm)
Instrument used for spectrophotometric analysis (UV-160A Shimadzu)
Calibration curve obtained using standard solutions of pure cyanidin-3 glucoside
Extinction coefficient used for calculating anthocyanin concentrations (1018.3)
Measurement performed in triplicate

positive controls

None mentioned

negative controls

None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!