Locomotion Analysis of Zebrafish Larvae

The locomotion analysis of 5 dpf zebrafish larvae was carried out by using the DanioVision system (Noldus Information Technology, Wageningen, Netherlands). The confocal imaging was performed by using a TCS-SP8 LSM confocal imaging system (Leica, Wetzlar, Germany). The zebrafish embryos were embedded as we previously did.^{37 (link)} Photographs of in situ hybridization results were taken by using a DP70 camera on an Olympus stereomicroscope MVX10. Statistical comparisons of the data were carried out by Student’s t-test, and P < 0.05 was considered statistically significant.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Li N., Zhou P., Tang H., He L., Fang X., Zhao J., Wang X., Qi Y., Sun C., Lin Y., Qin F., Yang M., Zhang Z., Liao C., Zheng S., Peng X., Xue T., Zhu Q., Li H., Li Y., Liu L., Huang J., Liu L., Peng C., Kaindl A.M., Gecz J., Han D., Liu D., Xu K, & Hu H. (2021). In-depth analysis reveals complex molecular aetiology in a cohort of idiopathic cerebral palsy. Brain, 145(1), 119-141.

Publication 2021

DanioVision system TCS-SP8 LSM confocal imaging system MVX10

Embryos In situ hybridization Larvae Locomotion Student Zebrafish

Corresponding Organization : Third Affiliated Hospital of Zhengzhou University

Other organizations : Tongji University, Charité - Universitätsmedizin Berlin, University of Adelaide

Top 5 similar protocols

Variable analysis

independent variables

Locomotion of 5 dpf zebrafish larvae

dependent variables

Locomotion analysis of 5 dpf zebrafish larvae using the DanioVision system
Confocal imaging of zebrafish embryos using a TCS-SP8 LSM confocal imaging system
Photographs of in situ hybridization results using a DP70 camera on an Olympus stereomicroscope MVX10

control variables

Zebrafish embryos embedded as previously done (reference 37)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!