Quantitative RNA Expression Analysis

Total RNA was extracted by RNApure Plant Kit (CWBIO) according to the manufacturer’s protocol. cDNA was reverse-transcribed using PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa). SYBR Premix Ex Taq II (TaKaRa) was used for qPCR on a ABI StepOne Plus real-time system (Life Technologies). qRT-PCR was performed in triplicate and data were collected and analyzed with ABI STEPONETM software version 2.1 [121 (link)]. Various gene specific signal was normalized relative to ACTIN2 gene (At3G18780) expression. The primer sequences were listed as follows:

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Wei D., Liu M., Chen H., Zheng Y., Liu Y., Wang X., Yang S., Zhou M, & Lin J. (2018). INDUCER OF CBF EXPRESSION 1 is a male fertility regulator impacting anther dehydration in Arabidopsis. PLoS Genetics, 14(10), e1007695.

Publication 2018

RNApure Plant Kit PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) SYBR Premix Ex Taq II ABI StepOne Plus real-time system

Abi 1 Abi 2 Cdna Gene Primer

Corresponding Organization : China Agricultural University

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Variable analysis

independent variables

None specified

dependent variables

Various gene-specific signal

control variables

ACTIN2 gene (At3G18780) expression

controls

Positive control: None specified
Negative control: None specified

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