Ferrostatin-1 (347174-05-4), liproxstatin-1 (950455-15-9), trolox (53188-07-1), deferoxamine (138-14-7), RSL-3 (1219810-16-8), erastin (571203-78-6), sulfasalazine (599-79-1), atorvastatin (599-79-1), KU60019 (925701-46-8) were purchased from Cayman. The concentration used is shown in Supplementary Table. S1 . C11BODIPY (D3861) was purchased from Invitrogen. Mouse recombinant IFNγ (485-MI-100), anti-mouse IFNγ block antibody (MAB485), human recombinant IFNγ (285-IF-100) were purchased from R&D. Anti-mouse IFNGR1 antibody (16-1193-85) was purchased from Thermo Fisher. The following antibody were used for immunoblots: anti-human SLC7A11 antibody (12691), anti-β-actin (12262), anti-GAPDH (2118), anti-STAT1 (9172), anti-phospho-STAT1 (9167), anti-ATM (2873) and anti-ACSL4 (4047) were bought from Cell Signaling Technology. Anti-GPX4 (ab41787) was from Abcam. Additionally, IgG (BE0087, BE0090), anti-mouse CTLA4 (BE0131), anti-mouse PD-L1 (BE0101) and anti-mouse CD8 (BE0117) were purchased from BioXcell. 4-HNE antibody (ab46545) was purchased from Abcam. Cyst(e)inase was generated as previously described (18 (link)).
Protocol detail
Ferroptosis-Inhibition Compound Evaluation
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Actin
Anti antibody
Antibody
Atorvastatin
Cayman
Ctla4
Cyst
Deferoxamine
Erastin
Ferrostatin 1
Gapdh
Gpx4
Human
Ifnγ
Immunoblots
Ku60019
Liproxstatin 1
Mouse
Mouse ifnγ
Pd l1
Stat1
Sulfasalazine
Trolox
Corresponding Organization : University of Michigan–Ann Arbor
Other organizations : Michigan Medicine, Michigan Center for Translational Pathology, The University of Texas at Austin, Howard Hughes Medical Institute
Top 5 similar protocols
Variable analysis
independent variables
- Ferrostatin-1 (347174-05-4)
- Liproxstatin-1 (950455-15-9)
- Trolox (53188-07-1)
- Deferoxamine (138-14-7)
- RSL-3 (1219810-16-8)
- Erastin (571203-78-6)
- Sulfasalazine (599-79-1)
- Atorvastatin (599-79-1)
- KU60019 (925701-46-8)
- Mouse recombinant IFN_γ (485-MI-100)
- Anti-mouse IFN_γ block antibody (MAB485)
- Human recombinant IFN_γ (285-IF-100)
- Anti-mouse IFNGR1 antibody (16-1193-85)
- IgG (BE0087, BE0090)
- Anti-mouse CTLA4 (BE0131)
- Anti-mouse PD-L1 (BE0101)
- Anti-mouse CD8 (BE0117)
- Concentration used (as shown in Supplementary Table. S1)
- C11BODIPY (D3861)
- Anti-human SLC7A11 antibody (12691)
- Anti-β-actin (12262)
- Anti-GAPDH (2118)
- Anti-STAT1 (9172)
- Anti-phospho-STAT1 (9167)
- Anti-ATM (2873)
- Anti-ACSL4 (4047)
- Anti-GPX4 (ab41787)
- 4-HNE antibody (ab46545)
- Cyst(e)inase (generated as previously described)
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