Genomic DNA was extracted from fungal mycelia grown on PDA, using a modified CTAB protocol as described in Guo et al. (2000) . Five loci, including the 5.8S nuclear ribosomal RNA gene with the two flanking internal transcribed spacer (ITS), translation elongation factor (EF-1α), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1) and partial RNA polymerase second largest subunit (RPB2) gene regions, were amplified and sequenced, respectively. The primer pairs and PCR amplification procedures following protocols described by Crous et al. (2009) (link) are listed in Table 2. PCR amplifications were performed in a reaction mixture consisting of 12.5 μL 2 × Taq PCR Master Mix (Vazyme Biotech Co., Ltd, Nanjing, China), 1 μL each of 10 μM primers, 1 μL of the undiluted genomic DNA, adjusted to a final volume of 25 μL with distilled deionized water. The PCR products were visualised on 1 % agarose electrophoresis gel. Sequencing was done bi-directionally, conducted by the TIANYI HUIYUAN Company (Beijing, China). Consensus sequences were obtained using SeqMan of the Lasergene soft-ware package v. 14.1 (DNAstar, Madison, Wisconsin, USA).