Isolation and Culture of EBV-associated Cells
Corresponding Organization : German Center for Infection Research
Other organizations : Frauenklinik Heidelberg, University Hospital Heidelberg, Helmholtz Zentrum München, Technical University of Munich
Variable analysis
- Cell lines used: EBV-positive Raji cells (ATCC CCL-86), EBV-negative Elijah B cells, 293 cells (ATCC CRL-1573), T cells specific for EBNA1 3E10, EBNA3C 5H11, gp350 1D6 and BNRF1 VSD epitopes, and autologous LCLs
- Not explicitly mentioned
- RPMI containing 10% fetal calf serum (F7524, Sigma) used to culture 293, Raji and Elijah cells
- T-cell clones and lines cultured as previously described [17,35]
- T cells specific for EBNA1 3E10, EBNA3C 5H11, gp350 1D6 and BNRF1 VSD epitopes
- EBV-negative Elijah B cells
Annotations
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