DNA Nanoparticle Self-Assembly Protocol

Self-assembly of DNA sequences of triangular nanoparticles was designed following our previous report [39 (link)]. Equimolar concentrations of individual strands (1 µM) were mixed in presence of various concentrations of conjugated ODNs in TMS buffer. To facilitate hybridization, the solution mixture of DNAs was heated to 80 °C for 5 min and slowly cooled down to 4 °C at a rate of 1 °C/min. An equal volume of loading buffer (sucrose 40% (wt/vol), 0.1% (wt/vol) xylene cyanol, and 0.1% (wt/vol) bromophenol blue) was added to each sample with subsequent analysis on 6% native PAGE in TBM. The gels were run at 60 V for 60 min at room temperature and imaged using ChemiDoc XRS (BioRad, Hercules, CA, USA) or Amersham Typhoon-5 (Cytiva, Marlborough, MA, USA) imaging systems with and without the presence of ethidium bromide.