Soil Elemental Analysis Protocol

The dry sandy soil samples were sieved with a metal sieve (mesh size: 2 mm) and homogenized by grinding them in a ball mill (Retsch, Haan, Germany). We weighed about 20 mg of ground dry material into tin capsules and analysed C and N in the samples with a vario MICRO cube elemental analyser (Elementar Analysensysteme GmbH). We used acetanilide (C 8 H 9 NO, HEKAtech GmbH, Wegberg, Germany) as the standard.

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Audisio M., Reulen H., Schott I., Paligi S., Mrak K., Hertel D., Leuschner C., & Polle A. (2024). Mycorrhization, root tip vitality and biomass of Fagus sylvatica, Picea abies and Pseudotsuga menziesii in monospecific and mixed combinations under water reduction and nitrogen addition. Trees, 38(3), 695-708.

Publication 2024

Corresponding Organization : University of Göttingen

Other organizations : Nordwestdeutsche Forstliche Versuchsanstalt

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Variable analysis

independent variables

Mesh size of metal sieve (2 mm)

dependent variables

Concentrations of C and N in the samples

control variables

Weight of ground dry material (about 20 mg)
Use of a ball mill (Retsch, Haan, Germany) to homogenize the samples
Use of acetanilide (C8H9NO, HEKAtech GmbH, Wegberg, Germany) as the standard

controls

Positive control: Acetanilide (C8H9NO, HEKAtech GmbH, Wegberg, Germany)
Negative control: Not specified

Annotations

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