Ovalbumin (OVA; grade V, ≥98% purity) and cow’s milk protein (CMP) rich in casein (87.1% [10 (link)]) were obtained from Sigma Chemical (Sigma-Aldrich, Saint Louis, MO, USA) and MP Biomedicals (Oklahoma, OH, USA), respectively. The test proteins were dissolved in PBS pH 7.4 (Sigma-Aldrich, Saint Louis, MO, USA; Cat: P3813), and the protein concentrations were determined (BCA assay, Thermo Scientific, Rockford, IL, USA).
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