Cloning and Analysis of BivCaE cDNA

The cDNA cloning of BivCaE cDNA was carried out using the B. ignitus cDNA library as previously described [20 (link)]. Plasmid DNA extraction was performed using the Wizard Mini-Prep Kit (Promega, Madison, WI, USA). The cDNA sequence was analyzed using the Basic Local Alignment Search Tool (BLAST) programs from National Center for Biotechnology Information (NCBI) [28 ]. The deduced protein sequences of BivCaE were aligned using MacVector (ver. 6.5, Oxford Molecular Ltd., Oxford, UK). The prediction of the signal sequence of BivCaE was carried out by the SignalP 4.1 Server [29 ]. The protein sequence of BivCaE was registered in GenBank (accession number MW699017).

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Deng Y., Kim B.Y., Lee K.Y., Yoon H.J., Wan H., Li J., Lee K.S, & Jin B.R. (2021). Lipolytic Activity of a Carboxylesterase from Bumblebee (Bombus ignitus) Venom. Toxins, 13(4), 239.

Publication 2021

Wizard Mini-Prep Kit

Cdna Cdna library Plasmid Promega Protein sequence Signal sequence

Corresponding Organization : Dong-A University

Other organizations : National Academy of Agricultural Science, Huazhong Agricultural University

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Variable analysis

independent variables

CDNA cloning of BivCaE cDNA

dependent variables

CDNA sequence analysis using BLAST programs
Deduced protein sequence alignment using MacVector
Prediction of signal sequence of BivCaE using SignalP 4.1 Server
Protein sequence of BivCaE registered in GenBank

control variables

B. ignitus cDNA library used for cDNA cloning
Wizard Mini-Prep Kit used for plasmid DNA extraction

controls

No positive or negative controls explicitly mentioned.

Annotations

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