APAP-Induced Acute Liver Injury in Mice

The mouse model of APAP induced acute liver injury was performed as previously described (You 2013 (link)). The mice were fasted overnight for approximately 16 h prior to intraperitoneal (i.p.) injection of phosphate buffered solution (PBS) or APAP (300 mg/kg, dissolved in warm PBS, Sigma, USA) at 9 a.m. The samples were collected after 8 h at 5 p.m. and 24 h at 9 a.m. next day. Blood was collected by retro-orbital puncture. Sera samples were collected for measurement of serum ALT and aspartate aminotransferase (AST) levels using an automated chemistry analyzer (Beckman AU5800, BECKMAN COULTER, USA). Liver samples were obtained at various time points after APAP challenge and paraffin-embedded tissue sections were prepared and stained with H&E. The Src family kinase selective inhibitor PP2 (Selleck, China) was injected to the mice intraperitoneally (1.5 mg/kg, dissolved in ethanol) at half an hour prior to APAP-treatment.