Fluorescent Protein-Based Imaging of Cellular Markers

As primary antibodies we used the HPV16-L1 detecting antibody 16L1-312F (mouse monoclonal, diluted 1:200; [29 (link)]) and a rabbit polyclonal antibody raised against the V5-tag (diluted 1:5000; cat# ab9116, Abcam, Cambridge, UK). In addition, we employed GFP-Booster Atto488 (Chromotek, cat# gba488, Planegg-Martinsried, Germany) and RFP-Booster Atto594 (cat# rba594, Chromotek, Planegg-Martinsried, Germany), both diluted 1:200. As secondary antibodies we used AlexaFluor488 labelled donkey anti-mouse (cat# A-21202, Carlsbad, CA) and AlexaFluor594 labelled donkey anti-rabbit (cat# ab150064, Abcam). For transfection, we used the previously described plasmids encoding CD63-GFP [18 (link)], V5-OBSL1 [27 (link), 30 (link)] and CD151-RFP [31 (link)].