Quantifying Protein Levels and Glutarylation in Rat Cortex

All used reagents were of the highest purity grade available. Trimethyl ester of adipoyl phosphonate (TMAP) was synthesized according to (19 (link)). EDTA was purchased from Serva (Germany); methanol—from Merck (Germany); Triton-X 100, KH₂PO₄, and NaCl—from Panreac (Spain); glycerol—from MP Biomedicals, LLC (Santa Ana, CA, United States). NAD⁺ was obtained from Gerbu (Heidelberg, Germany), oxidized glutathione—from Calbiochem (La Jolla, CA, United States). All other reagents were of the highest purity available and obtained from Sigma-Aldrich (Helicon, Moscow, Russia). Deionized MQ-grade water was used to prepare solutions. The used antibodies are indicated in section “Western-Blotting Quantification of the Protein Levels of OADH, Sirtuin 3, and Sirtuin 5 and of Glutarylated Proteins in Rat Cerebral Cortex.”