Tocopherols Determination by HPLC

Tocopherols were determined by HPLC analysis according to (AbdElgawad et al., 2015 (link)). Tocopherols were extracted with hexane using the MagNALyser (Roche, Vilvoorde, Belgium; 1 min, 7000 rpm). The dried extract (CentriVap concentrator, Labconco, KS, United States) was resuspended in hexane, and tocopherols were separated and quantified by HPLC (Shimadzu, ‘s Hertogenbosch, The Netherlands) (normal phase conditions, Particil Pac 5 μm column material, length 250 mm, i.d. 4.6 mm). Dimethyl tocol (DMT) was used as internal standard (5 ppm). Data were analyzed with Shimadzu Class VP 6.14 software.

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de Sousa A., AbdElgawad H., Asard H., Pinto A., Soares C., Branco-Neves S., Braga T., Azenha M., Selim S., Al Jaouni S., Fidalgo F, & Teixeira J. (2017). Metalaxyl Effects on Antioxidant Defenses in Leaves and Roots of Solanum nigrum L.. Frontiers in Plant Science, 8, 1967.

Publication 2017

MagNALyser CentriVap concentrator HPLC Class VP 6.14 software

Hexane Hplc Tocol Tocopherols

Corresponding Organization : Beni-Suef University

Other organizations : Al Jouf University, Suez Canal University, King Abdulaziz University

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Variable analysis

independent variables

Tocopherols extraction method (hexane using MagNALyser)

dependent variables

Tocopherols concentration

control variables

Dimethyl tocol (DMT) used as internal standard (5 ppm)
HPLC conditions (normal phase, Particil Pac 5 μm column material, length 250 mm, i.d. 4.6 mm)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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