qPCR and Western blot analyses were performed as previously described [39 (link), 40 (link)]. The primer sequences are provided in the Supplementary Methods. Primary antibodies against SREBP-2 (ab72856, ab112046, ab30682; Abcam), FLAG (F3165; Sigma-Aldrich), ALDH1A1 (HPA002123; Sigma-Aldrich), c-Myc (5605; Cell Signaling Technology), CD44 (5640; Cell Signaling Technology), and GAPDH (5174; Cell Signaling Technology) were used.
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