Cell lines characterization for EGFR studies

DKMG cells were purchased from the DSMZ cell bank (Braunschweig, Germany). Lung cancer (H1299, H1975), myeloid leukemia (K562), breast cancer (MDA-435), normal breast (MCF-10A), and embryonic (HEK-293) cell lines were obtained from the ATCC. All cell lines were cultured in RPMI 1640 or DMEM medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, and 1% penicillin/streptomycin (all from Sartorius, Israel). Cells were maintained at 37 °C in humidified 5% CO₂ atmosphere. H1299, H1975, and DKMG cells were characterized by cell surface overexpression of EGFR [32 (link)]. DKMG cells are known to express two forms of EGFR—wild type (WT) and another carrying a mutation in the external region of the receptor (EGFR^vIII) [33 (link)]. H1975 cells carry a mutation in the internal domain of EGFR (L858R/T790M) [34 (link)].