OE33 and SK-GT-4 cell lines were purchased from the European Collection of Cell Cultures (ECACC, UK). All cell lines were cultured in RPMI 1640 with 10% FCS and 1mM L-Glutamine and Penicillin/Streptomycin at 37°C and 5% CO2. The SK-GT-4 pcmv and miR-145 cell lines were established as previously described [15 (link)].
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