Apoptosis Induction and Inhibition Assays

TRAIL was commercially supplied (SuperKiller, Enzo Life Sciences, Farmingdale, NY, USA) or produced from 293T cells as described^{30 (link)}. Caspase inhibitors (BD Pharmingen, San Diego, CA, USA) were: Z-VAD-FMK (general caspase inhibitor), Z-FA-FMK (negative control). Bcl-2, Bcl-xL inhibitors ABT-263 and WEHI-539 were purchased from Cayman Chemicals (Ann Arbor, MI, USA). FasL and N-acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich (MO, USA). NUTLIN-3a was purchased from MedChemExpress (NJ, USA). Doxycycline was purchased from Sigma-Aldrich (Cat. No: D9891). D-luciferin was purchased from Biotium (CA, USA). Chaetocin was purchased from two sources (C9492–1mg, Sigma-Aldrich, MO, USA) and (S8068, Selleckchem, Houston, TX, USA). The epigenetic tool library was constructed as described^{31 (link)}.