Cell apoptosis of transfected SCC-4 and SCC-9 cells was assayed employing the flow cytometer (BD Biosciences, Franklin Lakes, NJ), in the presence of Annexin V/PI double staining kit (Invitrogen). Cell samples were collected from 6-well plates via centrifugation, then stained in Binding Buffer and assayed with flow cytometry [18 (link)]. Each samples were assayed for more than triplicate.
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