Quantification of Oxidative Stress Proteins

Western blotting for Glutathione Peroxidase 1 (Gpx1) (ABCAM, Billerica, MA), GSTπ (MBL International, Woburn, MA), GSTμ (ABCAM, Cambridge, MA), Heme Oxygenase 1 (HO-1) Enzo Life Sciences, Farmingdale, NY, GCLC (Novus Biologicals, Littleton, CO), P62 (SQSTM1, Sequestosome 1) mouse monoclonal (ABCAM), LC3B (Novus Biologicals), anti-GAPDH (Millipore, Billerica, MA) was performed from 10 μg of liver extracts as previously described [10 (link), 26 (link)–28 (link)]. Quantification of expression of each protein was performed using ImageJ (NIH) and normalized to overall GAPDH expression.

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Petersen D.R., Saba L.M., Sayin V.I., Papagiannakopoulos T., Schmidt E.E., Merrill G.F., Orlicky D.J, & Shearn C.T. (2018). Elevated Nrf-2 responses are insufficient to mitigate protein carbonylation in hepatospecific PTEN deletion mice. PLoS ONE, 13(5), e0198139.

Publication 2018

Heme Oxygenase 1 (HO-1) anti-GAPDH

Biologicals Gapdh Gclc Glutathione peroxidase 1 Heme oxygenase 1 Liver extracts Mouse Novus Protein Sequestosome 1

Corresponding Organization : Oregon State University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of Glutathione Peroxidase 1 (Gpx1)
Expression of GSTπ
Expression of GSTμ
Expression of Heme Oxygenase 1 (HO-1)
Expression of GCLC
Expression of P62 (SQSTM1, Sequestosome 1)
Expression of LC3B

control variables

GAPDH expression

controls

Positive control: None mentioned
Negative control: None mentioned

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