Intestinal 3DG Absorption and GLP-1 Response

After overnight fasting, saline (control) or 3DG at 50 mg/kg was administered to the rats by gastric gavage. Blood samples from aorta abdominals were collected before (0 min) and after (15, 30, 60, and 120 min) 3DG administration for the measurements of GLP-1 levels. Plasma GLP-1 (total) concentrations were measured using the Multi-Species GLP-1 Total ELISA kit (Millipore, MA, USA). The area under the curve (AUC) (0–120 min) for GLP-1 was calculated for each group of rats. Duodenum tissues after 1 hr administration of 3DG (50 mg/kg) were obtained for the measurement of 3DG contents by high-performance liquid chromatography, as described previously [16 (link)].