Antibodies and Cell Lines for HSV-2 and Influenza Research

The antibodies used in this study are listed in Supplemental Table 1. The following cell lines were used: HaCaT keratinocytes (Addex Bio) and THP-1 (American Type Culture Collection [ATCC], TIB-202). The recombinant HSV-2 strain 333, which encodes the β-gal bene (HSV-2/Gal) under CMV promoter control, inserted between virus UL3 and UL4 genes, was provided by Patricia Spear and Richard Longnecker (Northwestern University, Evanston, Illinois, USA) (31 (link)). The stem of influenza hemagglutinin (Flu-HA) strain H1 1999 NC was expressed using the vector VRC-3925 (32 (link)) in 293F cells and was provided by M. Gray and L. Stamatatos (Fred Hutchinson Cancer Research Center, Seattle, Washington, USA). The HSV-2 proteins gB2, gC2, and gH2/gL2 were provided by G. Cohen and R. Eisenberg (University of Pennsylvania, Philadelphia, Pennsylvania, USA). The HSV-2 proteins UL19ud, UL25, and gD2 (33 (link)) were provided by Immune Design Corp.

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Chang A., Sholukh A.M., Wieland A., Jaye D.L., Carrington M., Huang M.L., Xie H., Jerome K.R., Roychoudhury P., Greninger A.L., Koff J.L., Cohen J.B., Koelle D.M., Corey L., Flowers C.R, & Ahmed R. (2022). Herpes simplex virus lymphadenitis is associated with tumor reduction in a patient with chronic lymphocytic leukemia. The Journal of Clinical Investigation, 132(18), e161109.

Publication 2022

HaCaT keratinocytes THP-1

Antibodies Cancer Cell lines Cells Genes Hemagglutinin Hsv 2 Keratinocytes Proteins Stem Strain Vector Virus

Corresponding Organization : Emory University

Other organizations : University of Washington, Fred Hutch Cancer Center, National Cancer Institute, Center for Cancer Research, Frederick National Laboratory for Cancer Research, Ragon Institute of MGH, MIT and Harvard

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Variable analysis

independent variables

Antibodies listed in Supplemental Table 1
Cell lines: HaCaT keratinocytes and THP-1
Recombinant HSV-2 strain 333 encoding the β-gal gene under CMV promoter control
Stem of influenza hemagglutinin (Flu-HA) strain H1 1999 NC expressed using the vector VRC-3925 in 293F cells
HSV-2 proteins: gB2, gC2, gH2/gL2, UL19ud, UL25, and gD2

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None specified
Negative control: None specified

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