Metabolite Profiling of Arabidopsis Roots

Frozen Arabidopsis roots were ground to a fine powder with liquid nitrogen and 1-g plant material was treated with 80% (v/v) methanol. Supernatants were collected after centrifugation and cleaned by membrane filtration (Chromafil O-20/15 MS). Samples were then analyzed by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) on a Velos LTQ System (Thermo Fisher Scientific, Waltham, MA, USA) as described in more detail by Moradtalab and co-workers (Moradtalab et al., 2018 (link)).

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Ganz P., Porras-Murillo R., Ijato T., Menz J., Straub T., Stührwohldt N., Moradtalab N., Ludewig U, & Neuhäuser B. (2022). Abscisic acid influences ammonium transport via regulation of kinase CIPK23 and ammonium transporters. Plant Physiology, 190(2), 1275-1288.

Publication 2022

Velos LTQ System

Arabidopsis Centrifugation Filtration Frozen High performance liquid chromatography Mass spectrometry Membrane Methanol Nitrogen Plant Powder Roots Workers

Corresponding Organization :

Other organizations : University of Hohenheim

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Variable analysis

independent variables

Treatment with 80% (v/v) methanol

dependent variables

Metabolite profile analyzed by UHPLC-MS

control variables

Frozen Arabidopsis roots
Ground to a fine powder with liquid nitrogen
1-g plant material used
Supernatants collected after centrifugation
Samples cleaned by membrane filtration (Chromafil O-20/15 MS)
UHPLC-MS analysis on a Velos LTQ System (Thermo Fisher Scientific, Waltham, MA, USA)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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