ChIP was carried out twice independently as previously reported (Rymen et al., 2019 (link)) with minor modifications. One gram of whole roots was harvested from 14-d-old WOX13gGFP in wox13-2 seedlings and frozen with liquid nitrogen. Samples were ground to a fine powder using a multibeads shocker (MB1200, Yasui Kikai) and the nuclear fraction was isolated after cross-linking for 10 min with 1% formaldehyde (Sigma) under vacuum. Chromatin was sheared at 5°C with a focused ultrasonicator (Covaris) with the following settings: duty cycle 5%, intensity 4, and cycles per burst 200 for 25 min (the first replicate) or duty cycle 10%, intensity 5, and cycles per burst 200 for 15 min (the second replicate). Sheared chromatin was immunoprecipitated using antibodies against GFP (ab290, Abcom). The isolated DNA was quantified with the Qubit dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) and 1–2 ng of DNA was used to make each ChIP-seq library. Libraries were prepared using the KAPA Hyper Prep Kit for Illumina (KK8502, KAPA Biosystems) and Illumina compatible adaptors (E7335, E7500, E7710, E7730, NEB). Libraries were pooled and 84-bp single-stranded sequences were obtained using Illumina NextSeq500 sequencer.