Culturing and Transfecting Cell Lines

293T (human embryonic kidney; ATCC CRL-3216), Vero E6 (African green monkey kidney; ATCC CRL-1586), LLC-MK2 (rhesus macaque kidney; ATCC CCL-7) and EpoNi/22.1 (Buettikofer’s epauletted fruit bat kidney, [44 (link)], kindly provided by M.A. Müller and C. Drosten) cell lines were obtained from collaborators. The cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; PAN Biotech) supplemented with 10% fetal bovine serum (FBS; PAN Biotech) as well as 1x penicillin and streptomycin from a 100x stock solution (PAN Biotech). The cell lines were cultivated at 37 °C and 5% CO₂ in humidified atmosphere. Transfection of 293T cells was achieved by calcium phosphate precipitation.

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Hoffmann M., Kaufmann S.V., Fischer C., Maurer W., Moldenhauer A.S, & Pöhlmann S. (2019). Analysis of Resistance of Ebola Virus Glycoprotein-Driven Entry Against MDL28170, An Inhibitor of Cysteine Cathepsins. Pathogens, 8(4), 192.

Publication 2019

Vero E6 DMEM FBS penicillin and streptomycin

293t cells African green monkey Atmosphere Calcium phosphate Cell lines Cells Eagle Embryonic Fruit Human Kidney Penicillin Rhesus macaque Streptomycin Transfection

Corresponding Organization : University of Göttingen

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Variable analysis

independent variables

Cell lines used: 293T (human embryonic kidney), Vero E6 (African green monkey kidney), LLC-MK2 (rhesus macaque kidney), and EpoNi/22.1 (Buettikofer's epauletted fruit bat kidney)

dependent variables

Not explicitly mentioned

control variables

Cell culture conditions: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1x penicillin and streptomycin, cultivated at 37 °C and 5% CO2 in humidified atmosphere
Transfection method: Calcium phosphate precipitation for 293T cells

controls

No positive or negative controls were explicitly mentioned in the provided information.

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