MG-63 and Saos-2 cells were provided by the China Center for Type Culture Collection as in our previous study (Shu et al., 2020 (link)). MG-63 and Saos-2 cells were cultured in RPMI‑1640 and McCoy’s 5A medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), respectively, supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1% L‑glutamine, and 1% penicillin‑streptomycin sulfate (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37 °C in a 5% CO2 humidity-controlled incubator (Chen et al., 2019 (link); Zhou et al., 2016 (link)).
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