Expected nucleotide diversity (π) for various chromosomes were estimated with sliding-window analysis by using TASSEL v5.0 as described [49 (link)]. Estimation of fixation index (FST) was based on Wright’s F statistic [50 (link)] with use of SVS v8.8.5 (Golden Helix, Inc., Bozeman, MT, USA, www.goldenhelix.com).
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