In an experimental study conducted during 2018-2020, semen was collected from 30 healthy men aged 18-35 years, who were referred to Emam Reza Hospital, Birjand University of Medical Sciences (Birjand, Iran). In accordance with the WHO guidelines, all samples were considered normal. 14
All participants were requested to abstain from ejaculation 2-3 days before sample collection. Semen with any abnormalities (sperm count and motility, morphology, pH, viscosity) were excluded from the experiment. The study was approved by the Ethics Committee of Shiraz University of Medical Sciences (IR.SUMS.REC.1396.S808).
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