Characterization of LCMV-specific CD8+ T cells

The following mAb were purchased from eBioscience or Biolegend and used in an appropriate combination of fluorochromes: CD8 (clone 53-6.7; eBioscience), PD-1 (clone J43; eBioscience), LAG-3 (clone eBioC9B7W; eBioscience), 2B4 (clone eBio244F4; eBioscience), CD11a (clone M17/4; eBioscience), Thy1.1 (clone HIS51; eBioscience), IFNγ (clone XMG1.2; Biolegend) and TNFα (clone MP6-XT22; Biolegend). All LCMV-specific peptides were synthesized by Bio-Synthesis Inc (Bio-Synthesis, Louisville, TX): GP276-286 (SGVENPGGYCL) and GP33-41 (KAVYNFATM) (52 (link)). p:MHC class I tetramer H-2D^b GP276 and GP33 were made and used as previously described (25 (link), 48 (link)).

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Condotta S.A., Khan S.H., Rai D., Griffith T.S, & Badovinac V.P. (2015). Poly-microbial sepsis increases susceptibility to chronic viral infection and exacerbates CD8+ T cell exhaustion. Journal of immunology (Baltimore, Md. : 1950), 195(1), 116-125.

Publication 2015

CD8 (clone 53-6.7; PD-1 (clone J43; CD11a (clone M17/4; Thy1.1 (clone HIS51; IFNγ (clone XMG1.2; TNFα (clone MP6-XT22;

Clone Fluorochromes Ifnγ Lcmv Mhc class i Peptides Synthesis Tetramer Tnfα

Corresponding Organization : University of Iowa

Other organizations : University of Minnesota Medical Center, Minneapolis VA Health Care System

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Variable analysis

independent variables

LCMV-specific peptides: GP276-286 (SGVENPGGYCL) and GP33-41 (KAVYNFATM)

dependent variables

LAG-3
CD11a
Thy1.1
IFNγ
TNFα

control variables

P:MHC class I tetramer H-2Db GP276 and GP33

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