Culturing Streptomyces scabiei and Mutant

Streptomyces scabiei 87–22 and its ΔcebR mutant [22 (link)] were routinely cultured in Tryptic Soy broth (TSB, 30 g l⁻¹, Sigma-Aldrich) or ISP2 (for 1 l: 4 g Yeast Extract, 10 g Malt Extract, 4 g Dextrose, pH 7.2) liquid media at 28 °C under shaking (180 r.p.m., New Brunswick Innova 44 incubator shaker). Modified thaxtomin defined medium (TDM) ([25 (link)], without l-Sorbose) was used as minimal medium, supplemented with maltose 0.5 % (Sigma-Aldrich) in which cellobiose (Carbosynth) and cellotriose (Megazyme) were added as inducers. Sucrose was purchased from Merck.

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Deflandre B., Stulanovic N., Planckaert S., Anderssen S., Bonometti B., Karim L., Coppieters W., Devreese B, & Rigali S. (2022). The virulome of Streptomyces scabiei in response to cello-oligosaccharide elicitors. Microbial Genomics, 8(1), 000760.

Publication 2022

Tryptic Soy broth Innova 44 incubator maltose Carbosynth Sucrose

Cellobiose Cellotriose Dextrose L sorbose Maltose Streptomyces scabiei Sucrose Tryptic soy broth Yeast

Corresponding Organization :

Other organizations : University of Liège, Ghent University

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Variable analysis

independent variables

Streptomyces scabiei 87–22 and its Δcebr mutant

dependent variables

Growth in Tryptic Soy broth (TSB) or ISP2 liquid media
Growth in modified thaxtomin defined medium (TDM) supplemented with maltose, cellobiose, and cellotriose

control variables

Temperature (28 °C)
Shaking speed (180 r.p.m.)
PH of ISP2 medium (7.2)

controls

Positive control: Streptomyces scabiei 87–22 wild-type strain
Negative control: Streptomyces scabiei 87–22 Δcebr mutant

Annotations

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