Evaluating TRAIL and SW IV-134 Cytotoxicity

All cell lines (OVCAR3, HeLa, HPAC, BxPC3, AsPC-1, HEK293T) used in the experiments were obtained from American Type Culture Collection (ATCC, Manassas, VA). Recombinant human TRAIL was purchased from Enzo Life Science (formerly BIOMOL, International, Farmingdale, NY). The sigma-2/SMAC drug conjugate SW IV-134 was synthesized as previously reported [40 (link)]. Cell viability was detected using luciferase-based readout (CellTiter-Glo, Promega, Madison, WI). Caspase activation was determined employing Caspase-Glo Assay System (Promega, Madison, WI). The pan-caspase inhibitor Z-VAD-FMK was purchased from Enzo Life Sciences (Ann Arbor, MI).

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Su Y., Tatzel K., Wang X., Belt B., Binder P., Kuroki L., Powell M.A., Mutch D.G., Hawkins W.G, & Spitzer D. (2016). Mesothelin's minimal MUC16 binding moiety converts TR3 into a potent cancer therapeutic via hierarchical binding events at the plasma membrane. Oncotarget, 7(21), 31534-31549.

Publication 2016

OVCAR3 BxPC3 AsPC-1 HEK293T Recombinant human TRAIL CellTiter-Glo Caspase-Glo Assay System Z-VAD-FMK

Assay Caspase Caspase inhibitor Cell lines Cell viability Drug Hela Human Luciferase Promega Sw iv 134 Trail Z vad fmk

Corresponding Organization :

Other organizations : Washington University in St. Louis, China Medical University

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Variable analysis

independent variables

Recombinant human TRAIL
SW IV-134 (sigma-2/SMAC drug conjugate)

dependent variables

Cell viability
Caspase activation

control variables

Cell lines (OVCAR3, HeLa, HPAC, BxPC3, AsPC-1, HEK293T) obtained from American Type Culture Collection (ATCC, Manassas, VA)
Pan-caspase inhibitor Z-VAD-FMK purchased from Enzo Life Sciences (Ann Arbor, MI)

positive controls

None specified

negative controls

None specified

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