Murine Model of Invasive Pulmonary Aspergillosis

The virulence of A. fumigatus Δfbx mutants and the corresponding complemented strains was tested in an established murine model for IPA [37 (link),38 (link),77 (link)]. In brief, female CD-1 mice were immunosuppressed with cortisone acetate (25 mg/mouse intraperitoneally; Sigma-Aldrich) on days -3 and 0. Mice were anesthetized and intranasally infected with 20 μl of a fresh suspension containing 10⁶ conidia. A control group was mock infected with PBS to monitor the influence of the immunosuppression. The health status was monitored at least twice daily for 14 days and moribund animals (defined by severe dyspnoea and/or severe lethargy) were sacrificed. Infections were performed with a group of 10 mice for each tested strain. Lungs from euthanized animals were removed, and fixed in formalin and paraffin-embedded for histopathological analyses according to standard protocols [39 (link),78 (link)].

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Jöhnk B., Bayram Ö., Abelmann A., Heinekamp T., Mattern D.J., Brakhage A.A., Jacobsen I.D., Valerius O, & Braus G.H. (2016). SCF Ubiquitin Ligase F-box Protein Fbx15 Controls Nuclear Co-repressor Localization, Stress Response and Virulence of the Human Pathogen Aspergillus fumigatus. PLoS Pathogens, 12(9), e1005899.

Publication 2016

cortisone acetate

Animals Conidia Cortisone acetate Dyspnoea Embedded paraffin Female Formalin Immunosuppression Infections Lethargy Lungs Mice Murine Strain Virulence

Corresponding Organization : Friedrich Schiller University Jena

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Variable analysis

independent variables

Genotype of A. fumigatus strain (wildtype vs. Δfbx mutant vs. complemented strain)

dependent variables

Virulence of A. fumigatus strains in a murine model of invasive pulmonary aspergillosis (IPA)
Survival of mice infected with different A. fumigatus strains

control variables

Mouse strain (female CD-1 mice)
Immunosuppression protocol (cortisone acetate treatment on days -3 and 0)
Route of infection (intranasal instillation of 20 μl of 10^6 conidia)
Observation period (14 days)

positive control

Mock-infected control group of mice (PBS only) to monitor the influence of immunosuppression

negative control

Not explicitly mentioned

Annotations

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