2-hydroxypropyl-β-cyclodextrin (HPβCD) and cholesterol were purchased from Sigma-Aldrich, bafilomycin was from Cayman Chemical, and DAPI nuclear stain was from Enzo Life Sciences. TFEB siRNA (Cat. No. SI00094969) and control siRNA (Cat. No. 1027280) were purchased from Qiagen. pMSCV-PIG, gag-pol, and VSVG plasmids were from Addgene and TFEB-3XFLAG plasmid was a generous gift from Dr. Marco Sardiello (Baylor College of Medicine, Houston, TX)
H4 cells stably transfected for the expression of α-syn-EmGFP (H4/α-syn-GFP) were generated as previously described [34 (link),35 (link)]. Human H4 neuroglioma (HTB-148, ATCC), H4/α-syn-GFP, and HEK-293T cells (CRL-11268, ATCC) were cultured in high glucose DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 1% PSQ, and maintained at 37°C and 5% CO2.
H4 cells stably transfected for the expression of α-syn-EmGFP (H4/α-syn-GFP) were generated as previously described [34 (link),35 (link)]. Human H4 neuroglioma (HTB-148, ATCC), H4/α-syn-GFP, and HEK-293T cells (CRL-11268, ATCC) were cultured in high glucose DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 1% PSQ, and maintained at 37°C and 5% CO2.
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