Facilitating Microbiome Transplantation and IL-10R Modulation

FMT was performed through oral gavage of mucus (first day) and faeces (second and third days) preparations from donor mice. This protocol facilitates the engraftment of the mucus-associated bacteria. Donor mice were untreated (normobiotic) donors, DSS-treated (dysbiotic) or treated with metronidazole (1 g/L), vancomycin (1 g/L), streptomycin (2 g/L) or a cocktail of the three antibiotics combined in order to deplete distinct taxa of bacteria. Mucus was scraped from colons, diluted in PBS and administered to recipients at 1:1 ratio. Faeces were collected, diluted in PBS (50 mg/ml) and administered to recipients by oral gavage (10 mg/mouse) one day after the end of acute DSS administration.
For in vivo IL-10R blockade, mice were injected intraperitoneally with 250 µg InVivoMAb anti-mouse IL-10R (BioXCell, clone 1B1.3 A), or its Isotype (BioXCell), daily for 4 days starting from one day before FMT treatment.