In this study, the model organism Ciona robusta, was formerly classified as Ciona intestinalis. Molecular studies have confirmed that C. intestinalis constitutes a compilation of species rather than a single species [55 (link),56 (link),57 (link),58 (link)].
C. robusta specimens were collected from Sciacca harbor (Sicily, Italy) and were acclimatized and maintained as reported in Arizza et al. [33 (link)]
Fragments of pharynx tissue (200 mg) were immediately soaked in RNAlater tissue collection solution (AMBION, Austin, TX, USA) and Total RNA extraction was performed as reported in in Arizza et al. [33 (link)].
C. robusta specimens were collected from Sciacca harbor (Sicily, Italy) and were acclimatized and maintained as reported in Arizza et al. [33 (link)]
Fragments of pharynx tissue (200 mg) were immediately soaked in RNAlater tissue collection solution (AMBION, Austin, TX, USA) and Total RNA extraction was performed as reported in in Arizza et al. [33 (link)].
Free full text: Click here
