Cotton Fiber RNA-seq Profiling

Total RNA was extracted from 1.0 g of cotton fiber of DCF, LCF and WCF using RNA Purification Kit according to the manufacturer’s protocol (Sangon Biotech, Shanghai, China). The RNA-seq sequencing and assembly were conducted by the MetWare Technologies Corporation (Wuhan, China). The Agilent 2100 BioAnalyzer was used to detect the fragment size and concentration of the library. The combined probe anchoring polymerization (CPAS) technique was used for sequencing, and the read length of 150 bp was obtained. The library was constructed and sequenced on the Illumina HiSeq 2500 platform [41 (link)]. Following the removal of low-quality sequence reads, clean reads were mapped to the reference genome sequence (https://www.cottongen.org (accessed on 14 January 2021)) using HISAT2 program.