Neuronal Differentiation of Human Mesenchymal Stem Cells

For neuronal differentiation, induction medium containing Neurobasal media (Gibco, USA), B27 supplement (Gibco, USA), EGF and FGF2 (10 ng/ml each) (PeproTech Asia), L-Glutamine (Gibco, USA) PenStrep (Gibco, USA) was used. The induction protocol was carried out for 12 days with media change on every 3^rd day. Another set of study group included induction of hMSCs using the above mentioned induction media cocktail for 8 days, followed by addition of BDNF (50 ng/ml) on 9^th day. After termination of induction period at 12 days in both the study groups, the cells were used for further experiments^{18 (link), 20 (link)}.

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Singh M., Kakkar A., Sharma R., Kharbanda O.P., Monga N., Kumar M., Chowdhary S., Airan B, & Mohanty S. (2017). Synergistic Effect of BDNF and FGF2 in Efficient Generation of Functional Dopaminergic Neurons from human Mesenchymal Stem Cells. Scientific Reports, 7, 10378.

Publication 2017

Neurobasal media B27 supplement EGF and FGF2 L-Glutamine PenStrep

Cells Fgf2 L glutamine Neuronal Supplement

Corresponding Organization :

Other organizations : All India Institute of Medical Sciences, Institute of Genomics and Integrative Biology

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Variable analysis

independent variables

Induction medium containing Neurobasal media, B27 supplement, EGF and FGF2 (10 ng/ml each), L-Glutamine, and PenStrep
Addition of BDNF (50 ng/ml) on 9th day for one set of the study group

dependent variables

Neuronal differentiation

control variables

Neurobasal media
B27 supplement
L-Glutamine
PenStrep

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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