Each participant provided a blood sample (9ml), which was collected in an EDTA tube for DNA extraction, when attending the baseline GS:SFHS clinical appointment. This sample was stored at -20°C for between 33 and 497 days. At this point, 100μl of the sample (approximately equivalent to two drops of blood) was blotted onto a Whatman FTA
® classic card (GE Healthcare; cat. no. WB120205) and DNA was extracted from the remaining sample using the Nucleon BACC3 Genomic DNA Extraction Kit (GE Healthcare; cat. no. RPN8512)
18 (link), following the manufacturer’s instructions. The Whatman FTA
® cards were stored at room temperature for between 5 and 10 years until DNA extraction for this project. DNA was extracted from the entire blood spot using the QIAamp DNA Investigator Kit (Qiagen; cat. no. 56504), following the manufacturer’s instructions. The concentration of the DNA samples was measured using a Qubit 2.0 fluorometer and the Qubit dsDNA HS assay (Thermo Fisher; cat. no. Q32854). Sample storage, DNA extraction and DNA quantification took place at the
Edinburgh Clinical Research Facility at the University of Edinburgh.
® classic card (GE Healthcare; cat. no. WB120205) and DNA was extracted from the remaining sample using the Nucleon BACC3 Genomic DNA Extraction Kit (GE Healthcare; cat. no. RPN8512)
18 (link), following the manufacturer’s instructions. The Whatman FTA
® cards were stored at room temperature for between 5 and 10 years until DNA extraction for this project. DNA was extracted from the entire blood spot using the QIAamp DNA Investigator Kit (Qiagen; cat. no. 56504), following the manufacturer’s instructions. The concentration of the DNA samples was measured using a Qubit 2.0 fluorometer and the Qubit dsDNA HS assay (Thermo Fisher; cat. no. Q32854). Sample storage, DNA extraction and DNA quantification took place at the
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